CAS No. 890405-51-3

Batch: Purity:99.95
3BDO Chemical Structure
Chemical Structure
Check the 3BDO product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 3-benzyl-5-[(2-nitrophenoxy)methyl]oxolan-2-one
CAS Number 890405-51-3
Molecular Formula

C18H17NO5

Molecular Weight (MW) 327.33
SMILES C1C(C(=O)OC1COC2=CC=CC=C2[N+](=O)[O-])CC3=CC=CC=C3
InChIKey AXPZIVKEZRHGAS-UHFFFAOYSA-N

Ordering Information

Biological Activity

3BDO is a butyrolactone derivative and small-molecule mTOR activator that targets FKBP1A. By activating the mTOR signaling pathway, 3BDO modulates downstream cellular responses, effectively suppressing autophagy in primary human umbilical vein endothelial cells (HUVECs). Furthermore, activation of this pathway prevents oxLDL-induced endothelial apoptosis, thereby promoting cell survival and maintaining cellular homeostasis. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 65 mg/mL (198.57 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 19.7 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.