CAS No. 25526-93-6

Batch: Purity:99.9
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3'-Fluoro-3'-deoxythymidine (Alovudine) Chemical Structure
Chemical Structure
Check the 3'-Fluoro-3'-deoxythymidine (Alovudine) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-[(2R,4S,5R)-4-fluoro-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
CAS Number 25526-93-6
Molecular Formula

C10H13FN2O4
Molecular Weight (MW) 244.22
SMILES CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)F
InChIKey UXCAQJAQSWSNPQ-XLPZGREQSA-N

Ordering Information

Biological Activity

3'-Fluoro-3'-deoxythymidine (Alovudine) is a nucleoside analog that acts as a potent inhibitor of DNA polymerase γ and reverse transcriptase. By inhibiting polymerase activity and impairing nucleic acid chain elongation, it disrupts cellular and viral DNA replication pathways. Consequently, Alovudine suppresses viral replication and serves as a useful biomarker of DNA synthesis for monitoring cellular proliferation.

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 49 mg/mL (200.63 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 40 mg/mL
Water 20 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.