CAS No. 132-32-1

Batch: Purity:98.70
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3-Amino-9-ethylcarbazole Chemical Structure
Chemical Structure
Check the 3-Amino-9-ethylcarbazole product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 132-32-1
Molecular Formula

C14H14N2
Molecular Weight (MW) 210.27
SMILES CC[N]1C2=CC=CC=C2C3=CC(=CC=C13)N

Ordering Information

Biological Activity

3-Amino-9-ethylcarbazole (AEC) is a chromogenic substrate for horseradish peroxidase (HRP) utilized in immunoperoxidase staining and immunohistochemistry applications. Enzymatic oxidation of AEC by HRP yields an alcohol-soluble red precipitate at antigenic target sites. This red deposit allows visualization of tissue antigens and facilitates sequential immunostaining protocols, as the chromogenic signal can be erased by alcohol washing to enable iterative rounds of biomarker labeling in tissue sections.[1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 42 mg/mL (199.74 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 21 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.