CAS No. 54-96-6

Batch: Purity:99.78
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3,4-Diaminopyridine Chemical Structure
Chemical Structure
Check the 3,4-Diaminopyridine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 54-96-6
Molecular Formula

C5H7N3
Molecular Weight (MW) 109.13
SMILES C1=CN=CC(=C1N)N

Ordering Information

Biological Activity

3,4-Diaminopyridine is a voltage-gated potassium channel blocker that acts predominantly on presynaptic potassium channels. By inhibiting outward potassium currents during membrane repolarization, it prolongs presynaptic action potentials. This prolongation enhances voltage-gated calcium channel opening, leading to increased presynaptic calcium influx and stimulated acetylcholine release at the neuromuscular junction. Consequently, it promotes presynaptic neurotransmitter exocytosis and enhances neuromuscular signal transmission. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 22 mg/mL (201.59 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 22 mg/mL
Water 7 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.