CAS No. 547-65-9
Batch:
Purity:
Chemical Structure
Check the
2-Methylenebutyrolactone
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| CAS Number | 547-65-9 | |
|---|---|---|
| Molecular Formula |
C5H6O2 |
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| Molecular Weight (MW) | 98.10 |
Biological Activity
2-Methylenebutyrolactone (Tulipalin A) is a naturally occurring lactone and cytotoxic agent that functions as an electrophilic Michael acceptor. It selectively alkylates sulfhydryl groups on functional cysteine residues of biological thiols and enzymes, depleting intracellular glutathione levels. This inhibition of sulfhydryl-dependent proteins disrupts cellular redox balance and downstream survival signaling, resulting in the suppression of cell proliferation and the induction of apoptotic cell death.
How to Prepare Stock Solution?
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Mass
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Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
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Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Note: Technical data last updated: Sep 1, 2026.