CAS No. 328-50-7

Batch: Purity:99.98
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2-Ketoglutaric acid (alpha-ketoglutarate) Chemical Structure
Chemical Structure
Check the 2-Ketoglutaric acid (alpha-ketoglutarate) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-oxopentanedioic acid
CAS Number 328-50-7
Molecular Formula

C5H6O5
Molecular Weight (MW) 146.10
SMILES C(CC(=O)O)C(=O)C(=O)O
InChIKey KPGXRSRHYNQIFN-UHFFFAOYSA-N

Biological Activity

2-Ketoglutaric acid (alpha-ketoglutarate) is a key tricarboxylic acid (TCA) cycle metabolic intermediate and an inhibitor of tyrosinase. It serves as a substrate for alpha-ketoglutarate dehydrogenase to yield succinyl-CoA, playing a central role in cellular energy metabolism. Beyond its metabolic function, alpha-ketoglutarate regulates downstream pathways including TOR/mTOR signaling to modulate cellular respiration, proliferation, and lifespan. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 29 mg/mL (198.49 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 29 mg/mL
Ethanol 29 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Chin RM, et al. Nature. 2014;510(7505):397-401.

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Note: Technical data last updated: Sep 1, 2026.