Sodium oxamate

Catalog No.S6871 Batch:S687104

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Technical Data

Formula

C2H2NNaO3
Molecular Weight 111.03 CAS No. 565-73-1
Solubility (25°C)* In vitro Water 22 mg/mL (198.14 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Sodium oxamate (SO, Aminooxoacetic acid, Oxamic acid) is an inhibitor of lactate dehydrogenase (LDH) that specificly inhibits LDH‑A. Sodium oxamate (SO) induces G2/M cell cycle arrest via downregulation of the CDK1/cyclin B1 pathway and promotes apoptosis through enhancement of mitochondrial ROS generation.
Targets
LDHA [1] ROS [2] CDK1 [2]
In vitro

Sodium oxamate, a specific inhibitor of LDH-A, enhances the suppressive effects of PARP inhibitors on ovarian cancer without BRCA mutations, remarkably promoting the inhibitory effects of PARP inhibitors on wild-type BRCA ovarian cancer cells.[3]
In vivo

Sodium oxamate reduces the growth of colorectal cancer in CRC mice, by restoring the down-regulated MMR functional proteins and attenuating chemotherapy resistance to oxaliplatin caused by C. tropicalis.[4]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    wild-type BRCA ovarian cancer cells (A2780 and SKOV3)

  • Concentrations

    50 nM

  • Incubation Time

    24 h

  • Method

    Cell proliferation is evaluated using a Cell Counting Kit-8 (CCK-8). SKOV3 cells and A2780 cells are seeded in a 96-well culture plates at a density of 2×105 cells/well and incubated with different concentrations of the drugs (oxamate, AG14361 and olaparib) for different times. Detection reagent is added to the cells, and the luminescence signals are determined.
Animal Study:

[4]
  • Animal Models

    four-week-old male BALB/c nude mice of CRC xenograft

  • Dosages

    500 mg/kg

  • Administration

    i.p.

Selleck's Sodium oxamate has been cited by 8 publications

GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression [ J Exp Clin Cancer Res, 2024, 43(1):105] PubMed: 38576043
HSPA12A acts as a scaffolding protein to inhibit cardiac fibroblast activation and cardiac fibrosis [ J Adv Res, 2024, S2090-1232(24)00025-0] PubMed: 38219869
IL-6-Derived Autocrine Lactate Promotes Immune Escape of Uveal Melanoma [ Invest Ophthalmol Vis Sci, 2024, 65(3):37] PubMed: 38551584
Hepatocyte HSPA12A inhibits macrophage chemotaxis and activation to attenuate liver ischemia/reperfusion injury via suppressing glycolysis-mediated HMGB1 lactylation and secretion of hepatocytes [ Theranostics, 2023, 13(11):3856-3871] PubMed: 37441587
Mitochondrial Fragmentation Promotes Inflammation Resolution Responses in Macrophages via Histone Lactylation [ Mol Cell Biol, 2023, 10.1080/10985549.2023.2253131] PubMed: 37807652
Mitochondrial Fragmentation Promotes Inflammation Resolution Responses in Macrophages via Histone Lactylation [ Mol Cell Biol, 2023, 43(10):531-546] PubMed: 37807652
C. tropicalis promotes chemotherapy resistance in colon cancer through increasing lactate production to regulate the mismatch repair system [ Int J Biol Sci, 2021, 17(11):2756-2769] PubMed: 34345205
LDH-A inhibitors as remedies to enhance the anticancer effects of PARP inhibitors in ovarian cancer cells [ Aging (Albany NY), 2021, 13(24):25920-25930] PubMed: 34919531

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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