UQCRFS1/RISP Antibody [P24M17] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat

Lane 1: Neuro-2a, Lane 2: HT55, Lane 3: REC-1

Usage Information

Dilution
WB1:1000 IP1:50

Application
WB, IP

Reactivity
Human, Mouse, Rat

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
23 kDa

Datasheet & SDS

Biological Description

Specificity
UQCRFS1/RISP Antibody [P24M17] detects endogenous levels of total UQCRFS1/RISP protein.

Clone
P24M17

Synonym(s)
Cytochrome b-c1 complex subunit 5; Rieske iron-sulfur protein; RISP; UQCRFS1

Background
UQCRFS1, known as the Rieske iron-sulfur protein or RISP, functions as the mobile extrinsic subunit of mitochondrial complex III (cytochrome bc1 complex) within the electron transport chain, facilitating electron shuttling from ubiquinol to cytochrome c during oxidative phosphorylation. The protein harbors a [2Fe-2S] cluster coordinated by eight cysteine and histidine residues within a folded extrinsic domain tethered by a single transmembrane helix, enabling conformational swinging between Qo and Qi sites of cytochrome b to execute the Q-cycle that translocates four protons per two electrons while preventing superoxide leakage under physiological potentials. During catalysis, reduced ubiquinol at the Qo site donates the first electron to the high-potential [2Fe-2S] cluster, which relays it to cytochrome c1 via histidine ligation, while the semiquinone intermediate bifurcates the second electron to bL and bH hemes, sustaining chain continuity and protonmotive force generation coupled to ATP synthesis. BCS1L chaperone imports the mitochondrial targeting sequence-cleaved precursor from the matrix, stabilizing the holoform through LYRM7-mediated Fe-S cluster insertion before membrane integration proximal to UQCRC1/UQCRC2 core subunits. In hematopoietic stem cells, UQCRFS1 deficiency disrupts quiescence by impairing TGF-β signaling coordination through SHP-1 interactions, elevating cycling indices and exhausting long-term repopulation capacity during serial transplantation. T cell receptor stimulation recruits UQCRFS1 to lipid rafts where complex III activity amplifies NFAT and NF-κB activation via localized ROS and calcium flux, with knockout diminishing IL-2 production and proliferation.

Background

UQCRFS1, known as the Rieske iron-sulfur protein or RISP, functions as the mobile extrinsic subunit of mitochondrial complex III (cytochrome bc1 complex) within the electron transport chain, facilitating electron shuttling from ubiquinol to cytochrome c during oxidative phosphorylation. The protein harbors a [2Fe-2S] cluster coordinated by eight cysteine and histidine residues within a folded extrinsic domain tethered by a single transmembrane helix, enabling conformational swinging between Qo and Qi sites of cytochrome b to execute the Q-cycle that translocates four protons per two electrons while preventing superoxide leakage under physiological potentials. During catalysis, reduced ubiquinol at the Qo site donates the first electron to the high-potential [2Fe-2S] cluster, which relays it to cytochrome c1 via histidine ligation, while the semiquinone intermediate bifurcates the second electron to bL and bH hemes, sustaining chain continuity and protonmotive force generation coupled to ATP synthesis. BCS1L chaperone imports the mitochondrial targeting sequence-cleaved precursor from the matrix, stabilizing the holoform through LYRM7-mediated Fe-S cluster insertion before membrane integration proximal to UQCRC1/UQCRC2 core subunits. In hematopoietic stem cells, UQCRFS1 deficiency disrupts quiescence by impairing TGF-β signaling coordination through SHP-1 interactions, elevating cycling indices and exhausting long-term repopulation capacity during serial transplantation. T cell receptor stimulation recruits UQCRFS1 to lipid rafts where complex III activity amplifies NFAT and NF-κB activation via localized ROS and calcium flux, with knockout diminishing IL-2 production and proliferation.

References
https://pubmed.ncbi.nlm.nih.gov/29243944/ https://pubmed.ncbi.nlm.nih.gov/33753493/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%