SOX10 Antibody [E22E2] | Primary Antibodies

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000-1:10000 IP1:30 IHC1:1000

Application
WB, IP, IHC

Reactivity
Mouse, Rat, Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
270 kDa 314 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
SOX10 Antibody [E22E2] detects endogenous levels of total SOX10 protein.

Clone
E22E2

Synonym(s)
INSP3R1; ITPR1; IP3 receptor isoform 1; IP3R 1; InsP3R1; Type 1 InsP3 receptor

Background
SOX10, a SOX E-group transcription factor, is a master regulator of neural crest cell fate, orchestrating their specification, survival, migration, and differentiation into melanocytes, Schwann cells, and oligodendrocytes via DNA binding and coactivator recruitment. It consists of a central HMG-box DNA-binding domain that bends DNA at specific motifs, an N-terminal dimerization domain, dual transactivation domains (acidic K2/TAM and potent C-terminal TA/TAC), and regulatory phosphorylation and acetylation sites that fine-tune its activity. SOX10 primarily acts by occupying enhancers and promoters as a monomer and synergizing with lineage-defining transcription factors, such as Pax3→Mitf for melanocytes, Krox20→Egr2 for Schwann cells, and Olig1/2→Myrf for oligodendrocytes, to trigger stage-specific gene expression cascades, enforced through self-regulatory and partner-dependent mechanisms. This ensures proper formation of neural crest-derived glial cells in both the peripheral and central nervous systems, as well as the development of the inner ear, enteric nervous system, and oligodendroglial maturation. Loss of SOX10 disrupts neural crest cell development, while mutations cause disorders like Waardenburg syndrome, PCWH, and increase melanoma risk; its overexpression in tumors promotes stemness and cancer progression, all modulated by intricate auto-regulation and partner interactions.

Background

SOX10, a SOX E-group transcription factor, is a master regulator of neural crest cell fate, orchestrating their specification, survival, migration, and differentiation into melanocytes, Schwann cells, and oligodendrocytes via DNA binding and coactivator recruitment. It consists of a central HMG-box DNA-binding domain that bends DNA at specific motifs, an N-terminal dimerization domain, dual transactivation domains (acidic K2/TAM and potent C-terminal TA/TAC), and regulatory phosphorylation and acetylation sites that fine-tune its activity. SOX10 primarily acts by occupying enhancers and promoters as a monomer and synergizing with lineage-defining transcription factors, such as Pax3→Mitf for melanocytes, Krox20→Egr2 for Schwann cells, and Olig1/2→Myrf for oligodendrocytes, to trigger stage-specific gene expression cascades, enforced through self-regulatory and partner-dependent mechanisms. This ensures proper formation of neural crest-derived glial cells in both the peripheral and central nervous systems, as well as the development of the inner ear, enteric nervous system, and oligodendroglial maturation. Loss of SOX10 disrupts neural crest cell development, while mutations cause disorders like Waardenburg syndrome, PCWH, and increase melanoma risk; its overexpression in tumors promotes stemness and cancer progression, all modulated by intricate auto-regulation and partner interactions.

References
https://pubmed.ncbi.nlm.nih.gov/34667088/ https://pubmed.ncbi.nlm.nih.gov/21908409/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%