Datasheet

Biological Description

Specificity	SOX10 Antibody [E22E2] detects endogenous levels of total SOX10 protein.
Background	SOX10, a SOX E-group transcription factor, is a master regulator of neural crest cell fate, orchestrating their specification, survival, migration, and differentiation into melanocytes, Schwann cells, and oligodendrocytes via DNA binding and coactivator recruitment. It consists of a central HMG-box DNA-binding domain that bends DNA at specific motifs, an N-terminal dimerization domain, dual transactivation domains (acidic K2/TAM and potent C-terminal TA/TAC), and regulatory phosphorylation and acetylation sites that fine-tune its activity. SOX10 primarily acts by occupying enhancers and promoters as a monomer and synergizing with lineage-defining transcription factors, such as Pax3→Mitf for melanocytes, Krox20→Egr2 for Schwann cells, and Olig1/2→Myrf for oligodendrocytes, to trigger stage-specific gene expression cascades, enforced through self-regulatory and partner-dependent mechanisms. This ensures proper formation of neural crest-derived glial cells in both the peripheral and central nervous systems, as well as the development of the inner ear, enteric nervous system, and oligodendroglial maturation. Loss of SOX10 disrupts neural crest cell development, while mutations cause disorders like Waardenburg syndrome, PCWH, and increase melanoma risk; its overexpression in tumors promotes stemness and cancer progression, all modulated by intricate auto-regulation and partner interactions.

Specificity

SOX10 Antibody [E22E2] detects endogenous levels of total SOX10 protein.

Background

SOX10, a SOX E-group transcription factor, is a master regulator of neural crest cell fate, orchestrating their specification, survival, migration, and differentiation into melanocytes, Schwann cells, and oligodendrocytes via DNA binding and coactivator recruitment. It consists of a central HMG-box DNA-binding domain that bends DNA at specific motifs, an N-terminal dimerization domain, dual transactivation domains (acidic K2/TAM and potent C-terminal TA/TAC), and regulatory phosphorylation and acetylation sites that fine-tune its activity. SOX10 primarily acts by occupying enhancers and promoters as a monomer and synergizing with lineage-defining transcription factors, such as Pax3→Mitf for melanocytes, Krox20→Egr2 for Schwann cells, and Olig1/2→Myrf for oligodendrocytes, to trigger stage-specific gene expression cascades, enforced through self-regulatory and partner-dependent mechanisms. This ensures proper formation of neural crest-derived glial cells in both the peripheral and central nervous systems, as well as the development of the inner ear, enteric nervous system, and oligodendroglial maturation. Loss of SOX10 disrupts neural crest cell development, while mutations cause disorders like Waardenburg syndrome, PCWH, and increase melanoma risk; its overexpression in tumors promotes stemness and cancer progression, all modulated by intricate auto-regulation and partner interactions.

Usage Information

Application

WB, IP, IHC

Dilution

WB	IP	IHC
1:1000-1:10000	1:30	1:1000

Reactivity

Mouse, Rat, Human

Source

Rabbit Monoclonal Antibody

MW

270 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/34667088/
https://pubmed.ncbi.nlm.nih.gov/21908409/

SOX10 Antibody [E22E2]

Biological Description

Usage Information

References

Application Data