Sec61B Antibody [E5N6] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IP1:100 IF1:200 - 1:800

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
12 kDa

Datasheet & SDS

Biological Description

Specificity
Sec61B Antibody [E5N6] detects endogenous levels of total Sec61B protein.

Clone
E5N6

Synonym(s)
Protein transport protein Sec61 subunit beta; SEC61B

Background
Sec61B, the non-essential β-subunit of the heterotrimeric Sec61 translocon (with Sec61Aα/Sec61G), forms a compact immunoglobulin-like β-barrel (~96 aa) that laterally anchors to Sec61Aα's cytosolic vestibule via hydrophobic interfaces, stabilizing the ER membrane protein-conducting channel without direct transmembrane domain contact or catalytic activity. While Sec61Aα executes co-translational TMD insertion into the ER bilayer, Sec61B's primary function lies in post-ER vesicle trafficking: it facilitates EGFR/Her2 nuclear shuttling through endosomal recycling to drive proliferation signaling, directs Gurken (Drosophila TGF-α homolog) Golgi-to-plasma membrane transport essential for follicular axis patterning, and regulates copper-transporting ATPase (ATP7A/B) membrane distribution for cellular metal homeostasis. Sec61B knockout proves viable with only mild ER stress, but critically disrupts EGFR-dependent oncogenesis (mammary/epidermal cancers) and morphogen gradient formation during oogenesis, revealing its moonlighting role as a trafficking adaptor rather than classical translocon component.

Background

Sec61B, the non-essential β-subunit of the heterotrimeric Sec61 translocon (with Sec61Aα/Sec61G), forms a compact immunoglobulin-like β-barrel (~96 aa) that laterally anchors to Sec61Aα's cytosolic vestibule via hydrophobic interfaces, stabilizing the ER membrane protein-conducting channel without direct transmembrane domain contact or catalytic activity. While Sec61Aα executes co-translational TMD insertion into the ER bilayer, Sec61B's primary function lies in post-ER vesicle trafficking: it facilitates EGFR/Her2 nuclear shuttling through endosomal recycling to drive proliferation signaling, directs Gurken (Drosophila TGF-α homolog) Golgi-to-plasma membrane transport essential for follicular axis patterning, and regulates copper-transporting ATPase (ATP7A/B) membrane distribution for cellular metal homeostasis. Sec61B knockout proves viable with only mild ER stress, but critically disrupts EGFR-dependent oncogenesis (mammary/epidermal cancers) and morphogen gradient formation during oogenesis, revealing its moonlighting role as a trafficking adaptor rather than classical translocon component.

References
https://pubmed.ncbi.nlm.nih.gov/20937808/ https://pubmed.ncbi.nlm.nih.gov/26411746/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%