Sec61B Antibody [E5N6]

Catalog No.: F5872

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Biological Description

Specificity Sec61B Antibody [E5N6] detects endogenous levels of total Sec61B protein.
Background Sec61B, the non-essential β-subunit of the heterotrimeric Sec61 translocon (with Sec61Aα/Sec61G), forms a compact immunoglobulin-like β-barrel (~96 aa) that laterally anchors to Sec61Aα's cytosolic vestibule via hydrophobic interfaces, stabilizing the ER membrane protein-conducting channel without direct transmembrane domain contact or catalytic activity. While Sec61Aα executes co-translational TMD insertion into the ER bilayer, Sec61B's primary function lies in post-ER vesicle trafficking: it facilitates EGFR/Her2 nuclear shuttling through endosomal recycling to drive proliferation signaling, directs Gurken (Drosophila TGF-α homolog) Golgi-to-plasma membrane transport essential for follicular axis patterning, and regulates copper-transporting ATPase (ATP7A/B) membrane distribution for cellular metal homeostasis. Sec61B knockout proves viable with only mild ER stress, but critically disrupts EGFR-dependent oncogenesis (mammary/epidermal cancers) and morphogen gradient formation during oogenesis, revealing its moonlighting role as a trafficking adaptor rather than classical translocon component.

Usage Information

Application WB, IP, IF Dilution
WB IP IF
1:1000 1:100 1:200 - 1:800
Reactivity Human, Mouse, Rat, Monkey
Source Rabbit Monoclonal Antibody MW 12 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/20937808/
  • https://pubmed.ncbi.nlm.nih.gov/26411746/

Application Data