PSF Antibody [M17A24] | Primary Antibodies

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:500-1:1000

Application
WB, IP, IHC, IF

Reactivity
Human, Mouse

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
80 kDa

Datasheet & SDS

Biological Description

Specificity
PSF Antibody [M17A24] detects endogenous levels of total PSF protein.

Clone
M17A24

Background
PSF (Polypyrimidine tract-binding protein-associated splicing factor) belongs to the DBHS family of nuclear proteins and functions as a multifunctional nucleic acid-binding regulator of gene expression. The protein contains two RNA recognition motifs, a DNA-binding domain, and an N-terminal proline/glutamine-rich region that mediates protein-protein interactions. PSF represses transcription of multiple oncogenic genes through direct binding to their regulatory regions via its DNA-binding domain, while RNA-binding domains form complexes with non-coding VL30 retroelement RNA to release PSF repression and reverse transcriptional silencing. PSF stimulates splicing and 3'-end cleavage of pre-mRNA in a manner dependent on RNA polymerase II C-terminal domain phosphorylation and transcriptional activator strength, with distinct RNA recognition motifs playing nonredundant roles in promoting efficient cotranscriptional processing of 5'-proximal introns. PSF coordinates with p54nrb/NonO in spliceosomal complexes and interacts with U5 snRNP stem-loop 1 to recruit tri-snRNP subunits, ensuring accurate intron removal. The protein anchors hyper-edited RNAs in paraspeckles through high-affinity binding to inosine-rich sequences, preventing their cytoplasmic export until NEAT1 dissociation or specific cleavage relieves retention. PSF binds directly to double-strand breaks via its N-terminal RGG box through the proline-rich domain and recruits RAD51D recombinase for homologous recombination repair. Increased PSF expression inhibits proliferation and colony formation while suppressing oncogenes like P450ssc and GAGE6.

Background

PSF (Polypyrimidine tract-binding protein-associated splicing factor) belongs to the DBHS family of nuclear proteins and functions as a multifunctional nucleic acid-binding regulator of gene expression. The protein contains two RNA recognition motifs, a DNA-binding domain, and an N-terminal proline/glutamine-rich region that mediates protein-protein interactions. PSF represses transcription of multiple oncogenic genes through direct binding to their regulatory regions via its DNA-binding domain, while RNA-binding domains form complexes with non-coding VL30 retroelement RNA to release PSF repression and reverse transcriptional silencing. PSF stimulates splicing and 3'-end cleavage of pre-mRNA in a manner dependent on RNA polymerase II C-terminal domain phosphorylation and transcriptional activator strength, with distinct RNA recognition motifs playing nonredundant roles in promoting efficient cotranscriptional processing of 5'-proximal introns. PSF coordinates with p54nrb/NonO in spliceosomal complexes and interacts with U5 snRNP stem-loop 1 to recruit tri-snRNP subunits, ensuring accurate intron removal. The protein anchors hyper-edited RNAs in paraspeckles through high-affinity binding to inosine-rich sequences, preventing their cytoplasmic export until NEAT1 dissociation or specific cleavage relieves retention. PSF binds directly to double-strand breaks via its N-terminal RGG box through the proline-rich domain and recruits RAD51D recombinase for homologous recombination repair. Increased PSF expression inhibits proliferation and colony formation while suppressing oncogenes like P450ssc and GAGE6.

References
https://pubmed.ncbi.nlm.nih.gov/16079199/ https://pubmed.ncbi.nlm.nih.gov/16024807/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%