Phospho-YAP (Ser127) Antibody [K8E8]

Catalog No.: F0352

    Home Primary Antibodies Phospho-YAP (Ser127) Antibody [K8E8]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:200
    1:625 - 1:2500
    Application
    WB, IP, IHC
    Reactivity
    Human, Mouse, Rat
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    65-78 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-YAP (Ser127) Antibody [K8E8] detects endogenous levels of total YAP protein only when it is phosphorylated at Ser127.
    Clone
    K8E8
    Synonym(s)
    Hippo; P-YAP; Yes; Yes-associated
    Background
    Phospho-YAP (Ser127) refers to the phosphorylation of the Yes-associated protein (YAP) at serine 127, a critical regulatory event in the Hippo signaling pathway that controls tissue growth and organ size. YAP is a transcriptional co-activator containing domains such as WW, PDZ-binding, coiled-coil, and an SH3-binding motif, which facilitate interactions with transcription factors and signaling proteins. Phosphorylation at Ser127 by LATS1/2 kinases creates a binding site for 14-3-3 proteins, resulting in the cytoplasmic retention of YAP and inhibition of its transcriptional activity by preventing nuclear translocation. This phosphorylation event serves as a switch that modulates YAP’s ability to regulate gene expression programs involved in cell proliferation, survival, and stemness. Moreover, Ser127 phosphorylation primes YAP for subsequent phosphorylation by casein kinases CK1δ/ε on an adjacent phosphodegron, leading to proteasomal degradation, thereby tightly controlling YAP protein levels. Dysregulation of YAP phosphorylation at Ser127 is implicated in cancer progression, tissue regeneration, and developmental disorders.
    References
    • https://pubmed.ncbi.nlm.nih.gov/20048001/
    • https://pubmed.ncbi.nlm.nih.gov/32390875/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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