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Biological Description
| Specificity | Phospho-YAP (Ser127) Antibody [K8E8] detects endogenous levels of total YAP protein only when it is phosphorylated at Ser127. |
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| Background | Phospho-YAP (Ser127) refers to the phosphorylation of the Yes-associated protein (YAP) at serine 127, a critical regulatory event in the Hippo signaling pathway that controls tissue growth and organ size. YAP is a transcriptional co-activator containing domains such as WW, PDZ-binding, coiled-coil, and an SH3-binding motif, which facilitate interactions with transcription factors and signaling proteins. Phosphorylation at Ser127 by LATS1/2 kinases creates a binding site for 14-3-3 proteins, resulting in the cytoplasmic retention of YAP and inhibition of its transcriptional activity by preventing nuclear translocation. This phosphorylation event serves as a switch that modulates YAP’s ability to regulate gene expression programs involved in cell proliferation, survival, and stemness. Moreover, Ser127 phosphorylation primes YAP for subsequent phosphorylation by casein kinases CK1δ/ε on an adjacent phosphodegron, leading to proteasomal degradation, thereby tightly controlling YAP protein levels. Dysregulation of YAP phosphorylation at Ser127 is implicated in cancer progression, tissue regeneration, and developmental disorders. |
Usage Information
| Application | WB, IP, IHC | Dilution |
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| Reactivity | Human, Mouse, Rat | ||||||||
| Source | Rabbit Monoclonal Antibody | MW | 65-78 kDa | ||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3 | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||
References
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