Phospho-PKCθ/PRKCQ (Thr538) Antibody [D2M12]

Application: Reactivity: Human

Usage Information

Dilution
WB1:250-1:500 IHC1:150-1:250 FCM1:80-1:125

Application
WB, IHC, FCM

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
41 kDa,42 kDa,80 kDa,82 kDa,84 kDa

Datasheet & SDS

Biological Description

Specificity
Phospho-PKCθ/PRKCQ (Thr538) Antibody [D2M12] detects endogenous levels of total PKCθ/PRKCQ protein only when it is phosphorylated at Thr538.

Clone
D2M12

Synonym(s)
PRKCT; PRKCQ; Protein kinase C theta type; nPKC-theta

Background
Phospho-PKCθ/PRKCQ (Thr538) is the activated form of protein kinase C theta (PKCθ), a key enzyme in T cells. PKCθ belongs to the novel PKC family and helps T cells respond to signals from their receptor. It has special regions called C1 domains that bind a molecule called diacylglycerol (DAG), which helps PKCθ move to the cell membrane when T cells are activated. The phosphorylation (a chemical modification) at threonine 538 (Thr538) in the activation loop of PKCθ is essential because it turns the enzyme "on," allowing it to perform its function. Without this phosphorylation, PKCθ cannot carry out its kinase activity, even though it can still move to the membrane. This shows that membrane location and enzyme activation are controlled separately. When active, PKCθ helps activate important pathways that control T cell growth, division, and immune responses. It also plays a role in diseases like autoimmune disorders when its activity is not properly regulated.

Background

Phospho-PKCθ/PRKCQ (Thr538) is the activated form of protein kinase C theta (PKCθ), a key enzyme in T cells. PKCθ belongs to the novel PKC family and helps T cells respond to signals from their receptor. It has special regions called C1 domains that bind a molecule called diacylglycerol (DAG), which helps PKCθ move to the cell membrane when T cells are activated. The phosphorylation (a chemical modification) at threonine 538 (Thr538) in the activation loop of PKCθ is essential because it turns the enzyme "on," allowing it to perform its function. Without this phosphorylation, PKCθ cannot carry out its kinase activity, even though it can still move to the membrane. This shows that membrane location and enzyme activation are controlled separately. When active, PKCθ helps activate important pathways that control T cell growth, division, and immune responses. It also plays a role in diseases like autoimmune disorders when its activity is not properly regulated.

References
https://pubmed.ncbi.nlm.nih.gov/22798961/ https://pubmed.ncbi.nlm.nih.gov/26528291/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%