Phospho-p70 S6K (Thr421 Ser424) Antibody [E5J12]

Catalog No.: F2333

For research use only.

    Home Primary Antibodies Phospho-p70 S6K (Thr421 Ser424) Antibody [E5J12]

    Application: Reactivity:

    Usage Information

    Dilution
    1:500 - 1:3000
    1:100
    Application
    WB, IHC, ELISA
    Reactivity
    Human, Mouse, Rat
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    59 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-p70 S6K (Thr421 Ser424) Antibody [E5J12] detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr421 and Ser424 respectively.
    Clone
    E5J12
    Synonym(s)
    STK14A; RPS6KB1; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A
    Background
    Phospho-p70 S6 kinase (Thr421/Ser424) refers to the phosphorylated form of p70S6K at threonine 421 and serine 424, residues located within its carboxy-terminal autoinhibitory domain. p70S6K, also called ribosomal S6 kinase 1 (S6K1), is a 70-kDa serine/threonine kinase belonging to the AGC kinase family and a key downstream effector of the PI3K/Akt/mTOR pathway. The full-length enzyme contains an N-terminal regulatory region, a catalytic kinase domain, and a C-terminal autoinhibitory segment that controls activity. p70S6K is ubiquitously expressed in mammalian tissues, with particularly high levels in metabolically active and proliferative cells, including fibroblasts, immune cells, and developing tissues. Phosphorylation of Thr421/Ser424 occurs in response to growth factors, cytokines such as GM-CSF, and mitogenic signals, and it relieves autoinhibition to permit subsequent phosphorylation at Thr389 and Thr229, critical steps for full activation. Functionally, phosphorylation at Thr421/Ser424 contributes to enhanced enzymatic activity of p70S6K, which in turn phosphorylates substrates such as ribosomal protein S6 and eIF4B, thereby promoting translation initiation, protein synthesis, cell cycle progression, growth, and proliferation. In hematopoietic cells like neutrophils, GM-CSF–induced phosphorylation at these residues integrates signals from both mTOR- and MAPK-dependent pathways, highlighting phospho-Thr421/Ser424 as a key regulatory checkpoint for S6K activation in inflammation, immune responses, and growth control.
    References
    • https://pubmed.ncbi.nlm.nih.gov/12740386/
    • https://pubmed.ncbi.nlm.nih.gov/19730801/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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