Datasheet

Biological Description

Specificity	Phospho-p70 S6K (Thr421 Ser424) Antibody [E5J12] detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr421 and Ser424 respectively.
Background	Phospho-p70 S6 kinase (Thr421/Ser424) refers to the phosphorylated form of p70S6K at threonine 421 and serine 424, residues located within its carboxy-terminal autoinhibitory domain. p70S6K, also called ribosomal S6 kinase 1 (S6K1), is a 70-kDa serine/threonine kinase belonging to the AGC kinase family and a key downstream effector of the PI3K/Akt/mTOR pathway. The full-length enzyme contains an N-terminal regulatory region, a catalytic kinase domain, and a C-terminal autoinhibitory segment that controls activity. p70S6K is ubiquitously expressed in mammalian tissues, with particularly high levels in metabolically active and proliferative cells, including fibroblasts, immune cells, and developing tissues. Phosphorylation of Thr421/Ser424 occurs in response to growth factors, cytokines such as GM-CSF, and mitogenic signals, and it relieves autoinhibition to permit subsequent phosphorylation at Thr389 and Thr229, critical steps for full activation. Functionally, phosphorylation at Thr421/Ser424 contributes to enhanced enzymatic activity of p70S6K, which in turn phosphorylates substrates such as ribosomal protein S6 and eIF4B, thereby promoting translation initiation, protein synthesis, cell cycle progression, growth, and proliferation. In hematopoietic cells like neutrophils, GM-CSF–induced phosphorylation at these residues integrates signals from both mTOR- and MAPK-dependent pathways, highlighting phospho-Thr421/Ser424 as a key regulatory checkpoint for S6K activation in inflammation, immune responses, and growth control.

Specificity

Phospho-p70 S6K (Thr421 Ser424) Antibody [E5J12] detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr421 and Ser424 respectively.

Background

Phospho-p70 S6 kinase (Thr421/Ser424) refers to the phosphorylated form of p70S6K at threonine 421 and serine 424, residues located within its carboxy-terminal autoinhibitory domain. p70S6K, also called ribosomal S6 kinase 1 (S6K1), is a 70-kDa serine/threonine kinase belonging to the AGC kinase family and a key downstream effector of the PI3K/Akt/mTOR pathway. The full-length enzyme contains an N-terminal regulatory region, a catalytic kinase domain, and a C-terminal autoinhibitory segment that controls activity. p70S6K is ubiquitously expressed in mammalian tissues, with particularly high levels in metabolically active and proliferative cells, including fibroblasts, immune cells, and developing tissues. Phosphorylation of Thr421/Ser424 occurs in response to growth factors, cytokines such as GM-CSF, and mitogenic signals, and it relieves autoinhibition to permit subsequent phosphorylation at Thr389 and Thr229, critical steps for full activation. Functionally, phosphorylation at Thr421/Ser424 contributes to enhanced enzymatic activity of p70S6K, which in turn phosphorylates substrates such as ribosomal protein S6 and eIF4B, thereby promoting translation initiation, protein synthesis, cell cycle progression, growth, and proliferation. In hematopoietic cells like neutrophils, GM-CSF–induced phosphorylation at these residues integrates signals from both mTOR- and MAPK-dependent pathways, highlighting phospho-Thr421/Ser424 as a key regulatory checkpoint for S6K activation in inflammation, immune responses, and growth control.

Usage Information

Application

WB, IHC, ELISA

Dilution

WB	IHC
1:500 - 1:3000	1:100

Reactivity

Human, Mouse, Rat

Source

Rabbit Monoclonal Antibody

MW

59 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/12740386/
https://pubmed.ncbi.nlm.nih.gov/19730801/

Phospho-p70 S6K (Thr421 Ser424) Antibody [E5J12]

Biological Description

Usage Information

References

Application Data