Phospho-NEDD4-2 (Ser448) Antibody [D21H23]

Catalog No.: F3665

    Home Primary Antibodies Phospho-NEDD4-2 (Ser448) Antibody [D21H23]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000 - 1:5000
    1:10 - 1:100
    Application
    WB, IP
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    112 kDa 110-135 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-NEDD4-2 (Ser448) Antibody [D21H23] detects endogenous levels of total NEDD4-2 protein only when it is phosphorylated at Ser448.
    Clone
    D21H23
    Synonym(s)
    KIAA0439; NEDL3; NEDD4L; E3 ubiquitin-protein ligase NEDD4-like; HECT-type E3 ubiquitin transferase NED4L; NEDD4.2; Nedd4-2
    Background
    Phospho-NEDD4-2 (Ser448) is a phosphorylated form of NEDD4-2 (also known as NEDD4L), an E3 ubiquitin ligase belonging to the NEDD4 family characterized by an N-terminal calcium-dependent phospholipid-binding C2 domain, multiple WW protein-protein interaction domains, and a C-terminal catalytic HECT domain responsible for ubiquitin transfer. The WW domains recognize proline-rich PY motifs in substrate proteins such as epithelial sodium channels (ENaC), facilitating ubiquitination that targets these proteins for degradation, crucial for cellular homeostasis, especially in the kidney, lung, and colon tissues. Phosphorylation at Ser448 by AGC family kinases, including SGK1, Akt, and PKA, inhibits NEDD4-2's ligase activity, reducing ENaC ubiquitination and increasing sodium retention, with implications in hypertension and Liddle’s syndrome. The C2 domain mediates membrane targeting via Ca2+-dependent phospholipid binding, and autoinhibitory interactions involving C2, WW, and HECT domains regulate enzymatic activity through conformational modulation. NEDD4-2 also interacts with signaling pathways such as MAPK and Wnt, influencing proliferation, apoptosis, and tumorigenesis, where phospho-Ser448 modulates these functions by regulating substrate binding and ubiquitination dynamics.
    References
    • https://pubmed.ncbi.nlm.nih.gov/34294877/
    • https://pubmed.ncbi.nlm.nih.gov/22737130/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

    * Indicates a Required Field

    Please enter your name.
    Please enter your email. Please enter a valid email address.
    Please write something to us.