Phospho-NEDD4-2 (Ser448) Antibody [D21H23]

Catalog No.: F3665

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Biological Description

Specificity Phospho-NEDD4-2 (Ser448) Antibody [D21H23] detects endogenous levels of total NEDD4-2 protein only when it is phosphorylated at Ser448.
Background Phospho-NEDD4-2 (Ser448) is a phosphorylated form of NEDD4-2 (also known as NEDD4L), an E3 ubiquitin ligase belonging to the NEDD4 family characterized by an N-terminal calcium-dependent phospholipid-binding C2 domain, multiple WW protein-protein interaction domains, and a C-terminal catalytic HECT domain responsible for ubiquitin transfer. The WW domains recognize proline-rich PY motifs in substrate proteins such as epithelial sodium channels (ENaC), facilitating ubiquitination that targets these proteins for degradation, crucial for cellular homeostasis, especially in the kidney, lung, and colon tissues. Phosphorylation at Ser448 by AGC family kinases, including SGK1, Akt, and PKA, inhibits NEDD4-2's ligase activity, reducing ENaC ubiquitination and increasing sodium retention, with implications in hypertension and Liddle’s syndrome. The C2 domain mediates membrane targeting via Ca2+-dependent phospholipid binding, and autoinhibitory interactions involving C2, WW, and HECT domains regulate enzymatic activity through conformational modulation. NEDD4-2 also interacts with signaling pathways such as MAPK and Wnt, influencing proliferation, apoptosis, and tumorigenesis, where phospho-Ser448 modulates these functions by regulating substrate binding and ubiquitination dynamics.

Usage Information

Application WB, IP Dilution
WB IP
1:1000 - 1:5000 1:10 - 1:100
Reactivity Human
Source Rabbit Monoclonal Antibody MW 112 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/34294877/
  • https://pubmed.ncbi.nlm.nih.gov/22737130/

Application Data