Phospho-FAK (Tyr397) Antibody [G24K11]

Catalog No.: F2876

    Home Primary Antibodies Phospho-FAK (Tyr397) Antibody [G24K11]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:400
    Application
    WB, IF
    Reactivity
    Mouse, Rat, Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    119 kDa 119 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-FAK (Tyr397) Antibody [G24K11] detects endogenous levels of total FAK protein only when it is phosphorylated at Tyr397.
    Clone
    G24K11
    Synonym(s)
    FAK; FAK1; PTK2; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; p125FAK; pp125FAK; FRNK; PPP1R71
    Background
    Phospho-FAK (Tyr397) refers to the autophosphorylation of Focal Adhesion Kinase (FAK) at tyrosine 397, a critical regulatory event for FAK activation and function. FAK is a non-receptor tyrosine kinase composed of an N-terminal FERM (Four-point-one, Ezrin, Radixin, Moesin) domain, a central kinase domain, and a C-terminal focal adhesion targeting (FAT) domain, connected by linker regions containing key phosphorylation sites including Tyr397. In its inactive state, FAK’s FERM domain binds the kinase domain, sequestering Tyr397 and preventing autophosphorylation. Upon integrin engagement and extracellular matrix binding, conformational changes expose Tyr397, allowing autophosphorylation at this site, which triggers downstream signaling by recruiting Src family kinases and other effectors like PI3K and PLCγ. This phosphorylation event initiates further phosphorylation of additional tyrosine residues (e.g., Tyr576, Tyr577, Tyr861, Tyr925), enhancing FAK kinase activity and facilitating interactions with focal adhesion and cytoskeletal proteins, essential for cell adhesion, migration, survival, and proliferation. Phospho-Tyr397 acts as a docking site for SH2 domains of signaling proteins, linking FAK to pathways such as MAPK and PI3K/AKT, with critical implications in cancer progression, angiogenesis, and metastasis.
    References
    • https://pubmed.ncbi.nlm.nih.gov/17574028/
    • https://pubmed.ncbi.nlm.nih.gov/25625869/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

    * Indicates a Required Field

    Please enter your name.
    Please enter your email. Please enter a valid email address.
    Please write something to us.