Phospho-FAK (Tyr397) Antibody [G24K11]

Catalog No.: F2876

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Biological Description

Specificity Phospho-FAK (Tyr397) Antibody [G24K11] detects endogenous levels of total FAK protein only when it is phosphorylated at Tyr397.
Background Phospho-FAK (Tyr397) refers to the autophosphorylation of Focal Adhesion Kinase (FAK) at tyrosine 397, a critical regulatory event for FAK activation and function. FAK is a non-receptor tyrosine kinase composed of an N-terminal FERM (Four-point-one, Ezrin, Radixin, Moesin) domain, a central kinase domain, and a C-terminal focal adhesion targeting (FAT) domain, connected by linker regions containing key phosphorylation sites including Tyr397. In its inactive state, FAK’s FERM domain binds the kinase domain, sequestering Tyr397 and preventing autophosphorylation. Upon integrin engagement and extracellular matrix binding, conformational changes expose Tyr397, allowing autophosphorylation at this site, which triggers downstream signaling by recruiting Src family kinases and other effectors like PI3K and PLCγ. This phosphorylation event initiates further phosphorylation of additional tyrosine residues (e.g., Tyr576, Tyr577, Tyr861, Tyr925), enhancing FAK kinase activity and facilitating interactions with focal adhesion and cytoskeletal proteins, essential for cell adhesion, migration, survival, and proliferation. Phospho-Tyr397 acts as a docking site for SH2 domains of signaling proteins, linking FAK to pathways such as MAPK and PI3K/AKT, with critical implications in cancer progression, angiogenesis, and metastasis.

Usage Information

Application WB, IF Dilution
WB IF
1:1000 1:400
Reactivity Mouse, Rat, Human
Source Rabbit Monoclonal Antibody MW 119 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/17574028/
  • https://pubmed.ncbi.nlm.nih.gov/25625869/

Application Data