KPNB1 Antibody [K10N6]

Catalog No.: F1554

    Home Primary Antibodies KPNB1 Antibody [K10N6]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:5000
    1:1000
    1:100
    Application
    WB, IP, IF, FCM
    Reactivity
    Mouse, Rat, Human
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    97 kDa 97 kDa, 100 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    KPNB1 Antibody [K10N6] detects endogenous levels of total KPNB1 protein.
    Clone
    K10N6
    Synonym(s)
    NTF97; KPNB1; Importin subunit beta-1; Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97
    Background
    KPNB1, also known as importin beta, is a nucleocytoplasmic transport receptor in the karyopherin β family that mediates the import of proteins and ribonucleoproteins into the nucleus in a signal- and energy-dependent manner. As a solenoid protein, KPNB1 possesses the structural flexibility necessary for a wide range of interactions with cargo proteins and nuclear pore complex (NPC) components, specifically binding to FG-repeat motifs in nucleoporins to facilitate translocation. KPNB1 typically forms a complex with importin alpha, which recognizes nuclear localization signals (NLS) on cargo, while KPNB1 itself mediates interaction with the NPC and the Ran-GTPase system, ensuring directional, energy-dependent nuclear import. This transport pathway is essential for regulating signal transduction, cell cycle progression, and maintenance of cellular homeostasis. KPNB1 also interacts with NPC proteins such as NUP50 and NUP98, functioning as a gatekeeper to control cargo passage through the nuclear pore. Aberrant KPNB1 expression is associated with tumorigenesis and cancer progression, largely due to its role in importing oncogenic proteins like c-Myc and influencing transcriptional regulation.
    References
    • https://pubmed.ncbi.nlm.nih.gov/28427184/
    • https://pubmed.ncbi.nlm.nih.gov/37473981/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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