KPNB1 Antibody [K10N6]

Catalog No.: F1554

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Biological Description

Specificity KPNB1 Antibody [K10N6] detects endogenous levels of total KPNB1 protein.
Background KPNB1, also known as importin beta, is a nucleocytoplasmic transport receptor in the karyopherin β family that mediates the import of proteins and ribonucleoproteins into the nucleus in a signal- and energy-dependent manner. As a solenoid protein, KPNB1 possesses the structural flexibility necessary for a wide range of interactions with cargo proteins and nuclear pore complex (NPC) components, specifically binding to FG-repeat motifs in nucleoporins to facilitate translocation. KPNB1 typically forms a complex with importin alpha, which recognizes nuclear localization signals (NLS) on cargo, while KPNB1 itself mediates interaction with the NPC and the Ran-GTPase system, ensuring directional, energy-dependent nuclear import. This transport pathway is essential for regulating signal transduction, cell cycle progression, and maintenance of cellular homeostasis. KPNB1 also interacts with NPC proteins such as NUP50 and NUP98, functioning as a gatekeeper to control cargo passage through the nuclear pore. Aberrant KPNB1 expression is associated with tumorigenesis and cancer progression, largely due to its role in importing oncogenic proteins like c-Myc and influencing transcriptional regulation.

Usage Information

Application WB, IP, IF, FCM Dilution
WB IF FCM
1:5000 1:1000 1:100
Reactivity Mouse, Rat, Human
Source Mouse Monoclonal Antibody MW 97 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/28427184/
  • https://pubmed.ncbi.nlm.nih.gov/37473981/

Application Data