GLI1 Antibody [A19H14]

Catalog No.: F0381

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Biological Description

Specificity GLI1 Antibody [A19H14] detects endogenous levels of total GLI1 protein.
Background GLI1 (Glioma-associated oncogene homolog 1) is a zinc finger transcription factor and terminal effector of the Hedgehog (Hh) signaling pathway, originally identified as an amplified gene in malignant glioma. Structurally, GLI1 is a 1106 amino acid protein containing five tandem C2-H2 zinc finger DNA-binding domains that recognize the consensus sequence 5′-GACCACCCA-3′, a nuclear localization signal (NLS), nuclear export signal (NES), an N-terminal SUFU-interacting domain (SIN) that mediates cytoplasmic retention, and a C-terminal transactivation domain (TAD) that recruits chromatin remodelers and co-activators. GLI1 is expressed at low levels in differentiated tissues but is tightly regulated during embryonic development and tissue patterning, where it promotes cell differentiation and tissue polarity. Functionally, GLI1 acts as a strong transcriptional activator, regulating genes involved in cell cycle progression, DNA replication, and DNA damage repair, while its aberrant activation drives oncogenic processes including proliferation, survival, angiogenesis, metastasis, and chemoresistance in various cancers.

Usage Information

Application WB, IP Dilution
WB IP
1:1000 1:50
Reactivity Human
Source Rabbit Monoclonal Antibody MW 160 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/34113570/
  • https://pubmed.ncbi.nlm.nih.gov/22391302/

Application Data