Firefly Luciferase Antibody [H5E21]

Catalog No.: F2045

For research use only.

    Home Primary Antibodies Firefly Luciferase Antibody [H5E21]

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000 - 1:20000
    1:500
    1:70
    Application
    WB, IF, FCM
    Reactivity
    Transfected cell line - Firefly
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    61kDa

    Datasheet & SDS

    Biological Description

    Specificity
    Firefly Luciferase Antibody [H5E21] detects exogenously expressed proteins containing firefly luciferase.
    Clone
    H5E21
    Synonym(s)
    Luciferin 4-monooxygenase; Luciferase
    Background
    Firefly luciferase is a mono-oxygenase enzyme belonging to the acyl-adenylate/thioester-forming superfamily that catalyzes bioluminescence by oxidizing the substrate D-luciferin with ATP, Mg²⁺, and oxygen, producing oxyluciferin, CO₂, AMP, and yellow-green light. Structurally, it contains a catalytic domain that first adenylates luciferin to form luciferyl-AMP and then oxygenates it to yield the excited-state oxyluciferin responsible for light emission; it also shares homology with fatty acyl-CoA synthetases, reflecting its evolutionary origin. Firefly luciferase is expressed in specialized light-producing organs of luminous beetles such as fireflies (Lampyridae), railroad worms, and click beetles, but not in non-luminous insects. Functionally, beyond its natural role in bioluminescence for mating communication and signaling, the enzyme also exhibits a secondary catalytic activity of fatty acyl-CoA synthesis, highlighting its evolutionary link to acyl-CoA synthetases. In biotechnology, it is extensively used as a sensitive reporter enzyme to measure ATP levels and monitor gene expression in molecular and cell biology.
    References
    • https://pubmed.ncbi.nlm.nih.gov/19859663/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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