|
Toll Free: (877) 796-6397 -- USA and Canada only -- |
Fax: +1-832-582-8590 Orders: +1-832-582-8158 |
Tech Support: +1-832-582-8158 Ext:3 Please provide your Order Number in the email. |
Biological Description
| Specificity | Firefly Luciferase Antibody [H5E21] detects exogenously expressed proteins containing firefly luciferase. |
|---|---|
| Background | Firefly luciferase is a mono-oxygenase enzyme belonging to the acyl-adenylate/thioester-forming superfamily that catalyzes bioluminescence by oxidizing the substrate D-luciferin with ATP, Mg²⁺, and oxygen, producing oxyluciferin, CO₂, AMP, and yellow-green light. Structurally, it contains a catalytic domain that first adenylates luciferin to form luciferyl-AMP and then oxygenates it to yield the excited-state oxyluciferin responsible for light emission; it also shares homology with fatty acyl-CoA synthetases, reflecting its evolutionary origin. Firefly luciferase is expressed in specialized light-producing organs of luminous beetles such as fireflies (Lampyridae), railroad worms, and click beetles, but not in non-luminous insects. Functionally, beyond its natural role in bioluminescence for mating communication and signaling, the enzyme also exhibits a secondary catalytic activity of fatty acyl-CoA synthesis, highlighting its evolutionary link to acyl-CoA synthetases. In biotechnology, it is extensively used as a sensitive reporter enzyme to measure ATP levels and monitor gene expression in molecular and cell biology. |
Usage Information
| Application | WB, IF, FCM | Dilution |
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Reactivity | Transfected cell line - Firefly | ||||||||
| Source | Rabbit Monoclonal Antibody | MW | 61kDa | ||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3 | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||
References
|