Connexin 43 Antibody [F6F11]

Catalog No.: F3041

    Home Primary Antibodies Connexin 43 Antibody [F6F11]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:100
    1:200 - 1:800
    1:400 - 1:1600
    Application
    WB, IP, IHC, IF
    Reactivity
    Human, Mouse, Rat
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    39 kDa, 41 kDa, 43 kDa, 44 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    Connexin 43 Antibody [F6F11] detects endogenous levels of total Connexin 43 protein.
    Clone
    F6F11
    Synonym(s)
    Gap junction alpha-1 protein; Connexin-43 (Cx43); Gap junction 43 kDa heart protein; GJA1; GJAL
    Background
    Connexin 43 (Cx43), encoded by the GJA1 gene, is a fundamental gap junction protein belonging to the connexin family that assembles into hexameric structures known as connexons. These connexons are transported to the plasma membrane, where they dock with corresponding connexons from adjacent cells to form gap junction channels, facilitating direct intercellular communication essential for tissue homeostasis and synchronized cellular functions. Cx43 comprises four transmembrane domains, two extracellular loops (ECL1 and ECL2) essential for docking and gap junction channel formation, one cytoplasmic loop, and intracellular N- and C-terminal tails enriched with phosphorylation sites that regulate channel gating, assembly, and turnover. The C-terminal tail of Cx43 modulates interactions with kinases such as MAPK, PKC, and Src, whose phosphorylation events dynamically control gap junction permeability and stability. Cx43 channels allow the passage of ions, metabolites, and signaling molecules, playing a critical role in electrical and chemical coupling in excitable tissues like the heart and brain, thus ensuring proper cardiac contractility and neural communication. Cx43 hemichannels participate in paracrine signaling and mediate exchanges between the intracellular and extracellular environments. Dysregulation of Cx43 expression, phosphorylation, or mutations results in pathological conditions such as cardiac arrhythmias and various cancers, where altered intercellular communication contributes to disease progression and metastasis.
    References
    • https://pubmed.ncbi.nlm.nih.gov/35205149/
    • https://pubmed.ncbi.nlm.nih.gov/37535063/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

    * Indicates a Required Field

    Please enter your name.
    Please enter your email. Please enter a valid email address.
    Please write something to us.