Datasheet

Biological Description

Specificity	Connexin 43 Antibody [F6F11] detects endogenous levels of total Connexin 43 protein.
Background	Connexin 43 (Cx43), encoded by the GJA1 gene, is a fundamental gap junction protein belonging to the connexin family that assembles into hexameric structures known as connexons. These connexons are transported to the plasma membrane, where they dock with corresponding connexons from adjacent cells to form gap junction channels, facilitating direct intercellular communication essential for tissue homeostasis and synchronized cellular functions. Cx43 comprises four transmembrane domains, two extracellular loops (ECL1 and ECL2) essential for docking and gap junction channel formation, one cytoplasmic loop, and intracellular N- and C-terminal tails enriched with phosphorylation sites that regulate channel gating, assembly, and turnover. The C-terminal tail of Cx43 modulates interactions with kinases such as MAPK, PKC, and Src, whose phosphorylation events dynamically control gap junction permeability and stability. Cx43 channels allow the passage of ions, metabolites, and signaling molecules, playing a critical role in electrical and chemical coupling in excitable tissues like the heart and brain, thus ensuring proper cardiac contractility and neural communication. Cx43 hemichannels participate in paracrine signaling and mediate exchanges between the intracellular and extracellular environments. Dysregulation of Cx43 expression, phosphorylation, or mutations results in pathological conditions such as cardiac arrhythmias and various cancers, where altered intercellular communication contributes to disease progression and metastasis.

Specificity

Connexin 43 Antibody [F6F11] detects endogenous levels of total Connexin 43 protein.

Background

Connexin 43 (Cx43), encoded by the GJA1 gene, is a fundamental gap junction protein belonging to the connexin family that assembles into hexameric structures known as connexons. These connexons are transported to the plasma membrane, where they dock with corresponding connexons from adjacent cells to form gap junction channels, facilitating direct intercellular communication essential for tissue homeostasis and synchronized cellular functions. Cx43 comprises four transmembrane domains, two extracellular loops (ECL1 and ECL2) essential for docking and gap junction channel formation, one cytoplasmic loop, and intracellular N- and C-terminal tails enriched with phosphorylation sites that regulate channel gating, assembly, and turnover. The C-terminal tail of Cx43 modulates interactions with kinases such as MAPK, PKC, and Src, whose phosphorylation events dynamically control gap junction permeability and stability. Cx43 channels allow the passage of ions, metabolites, and signaling molecules, playing a critical role in electrical and chemical coupling in excitable tissues like the heart and brain, thus ensuring proper cardiac contractility and neural communication. Cx43 hemichannels participate in paracrine signaling and mediate exchanges between the intracellular and extracellular environments. Dysregulation of Cx43 expression, phosphorylation, or mutations results in pathological conditions such as cardiac arrhythmias and various cancers, where altered intercellular communication contributes to disease progression and metastasis.

Usage Information

Application

WB, IP, IHC, IF

Dilution

WB	IP	IHC	IF
1:1000	1:100	1:200 - 1:800	1:400 - 1:1600

Reactivity

Human, Mouse, Rat

Source

Rabbit Monoclonal Antibody

MW

39 kDa, 41 kDa, 43 kDa, 44 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/35205149/
https://pubmed.ncbi.nlm.nih.gov/37535063/

Connexin 43 Antibody [F6F11]

Biological Description

Usage Information

References

Application Data