Cleaved Caspase-3 Antibody [G2J3]

Catalog No.: F2523

For research use only.

    Home Primary Antibodies Cleaved Caspase-3 Antibody [G2J3]

    Application: Reactivity:

    Usage Information

    Dilution
    1:500
    1:100 - 1:250
    Application
    WB, IF
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Observed MW
    16 kDa/28 kDa, 17 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    Cleaved Caspase-3 Antibody [G2J3] detects endogenous levels of activated caspase-3.
    Clone
    G2J3
    Synonym(s)
    CPP32; CASP3; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; Protein Yama; SREBP cleavage activity 1; CPP-32; SCA-1
    Background
    Cleaved caspase-3 is the active form of caspase-3, a key executioner protease in apoptosis and a member of the Cysteine-ASPartic proteASES family (cysteine proteases). It is generated by proteolytic cleavage of procaspase-3 at specific aspartate residues, producing large (p17) and small (p12) subunits that assemble into a functional heterotetramer. This active enzyme is widely expressed across tissues but shows increased levels in pathological conditions such as cancer, where its expression often marks apoptotic activity. Functionally, cleaved caspase-3 orchestrates the execution phase of apoptosis by cleaving crucial substrates, including ICAD, PARP, and cytoskeletal proteins, thereby driving DNA fragmentation, chromatin condensation, and the characteristic morphological changes of apoptotic cells. Beyond apoptosis, emerging evidence also implicates cleaved caspase-3 in non-apoptotic roles, such as transcriptional regulation and angiogenesis, highlighting its dual role in cell death and tumor progression.
    References
    • https://pubmed.ncbi.nlm.nih.gov/35551578/
    • https://pubmed.ncbi.nlm.nih.gov/36233242/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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