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CD56 Antibody [C5A9]

Catalog No.: F1518

Application: Reactivity: Mouse

Usage Information

Dilution
WB1:900 IHC1:30-1:300 FCM0.25 µg

Application
WB, IHC, FCM

Reactivity
Mouse

Source
Rat Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
120-190 kDa 120 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
CD56 Antibody [C5A9] detects endogenous levels of total CD56 protein.

Clone
C5A9

Synonym(s)
CD56, NCAM1

Background
CD56, also known as neural cell adhesion molecule 1 (NCAM1), is a glycoprotein member of the immunoglobulin superfamily involved in mediating cell-cell adhesion and signaling. It generates multiple isoforms via alternative splicing, with molecular weights ranging from approximately 120 kDa (GPI-anchored), 140 kDa (short cytoplasmic domain), to 180 kDa (long cytoplasmic domain), the 140 kDa isoform being predominant on mature natural killer (NK) cells. CD56/NCAM1 contains five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats, which facilitate both homophilic and heterophilic interactions essential for neuronal attachment, neurite outgrowth, and immune cell function. Polysialylation of CD56 modulates its adhesive properties, contributing to enhanced tumor metastasis in cancers such as small-cell lung carcinoma and neuroblastoma. CD56 is a key marker of NK cells and subsets of T cells, regulating cytotoxic activity, cell migration, and immune surveillance. CD56 signals through kinases such as Fyn and focal adhesion kinase (FAK), modulating cytoskeletal dynamics critical for NK cell migration and function by influencing integrin-mediated adhesion turnover and actin remodeling.

Background

CD56, also known as neural cell adhesion molecule 1 (NCAM1), is a glycoprotein member of the immunoglobulin superfamily involved in mediating cell-cell adhesion and signaling. It generates multiple isoforms via alternative splicing, with molecular weights ranging from approximately 120 kDa (GPI-anchored), 140 kDa (short cytoplasmic domain), to 180 kDa (long cytoplasmic domain), the 140 kDa isoform being predominant on mature natural killer (NK) cells. CD56/NCAM1 contains five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats, which facilitate both homophilic and heterophilic interactions essential for neuronal attachment, neurite outgrowth, and immune cell function. Polysialylation of CD56 modulates its adhesive properties, contributing to enhanced tumor metastasis in cancers such as small-cell lung carcinoma and neuroblastoma. CD56 is a key marker of NK cells and subsets of T cells, regulating cytotoxic activity, cell migration, and immune surveillance. CD56 signals through kinases such as Fyn and focal adhesion kinase (FAK), modulating cytoskeletal dynamics critical for NK cell migration and function by influencing integrin-mediated adhesion turnover and actin remodeling.

References
https://pubmed.ncbi.nlm.nih.gov/30814062/ https://pubmed.ncbi.nlm.nih.gov/38507235/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%