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Biological Description
| Specificity | CD56 Antibody [C5A9] detects endogenous levels of total CD56 protein. |
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| Background | CD56, also known as neural cell adhesion molecule 1 (NCAM1), is a glycoprotein member of the immunoglobulin superfamily involved in mediating cell-cell adhesion and signaling. It generates multiple isoforms via alternative splicing, with molecular weights ranging from approximately 120 kDa (GPI-anchored), 140 kDa (short cytoplasmic domain), to 180 kDa (long cytoplasmic domain), the 140 kDa isoform being predominant on mature natural killer (NK) cells. CD56/NCAM1 contains five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats, which facilitate both homophilic and heterophilic interactions essential for neuronal attachment, neurite outgrowth, and immune cell function. Polysialylation of CD56 modulates its adhesive properties, contributing to enhanced tumor metastasis in cancers such as small-cell lung carcinoma and neuroblastoma. CD56 is a key marker of NK cells and subsets of T cells, regulating cytotoxic activity, cell migration, and immune surveillance. CD56 signals through kinases such as Fyn and focal adhesion kinase (FAK), modulating cytoskeletal dynamics critical for NK cell migration and function by influencing integrin-mediated adhesion turnover and actin remodeling. |
Usage Information
| Application | WB, IHC, FCM | Dilution |
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| Reactivity | Mouse | ||||||||
| Source | Rat Monoclonal Antibody | MW | 120-190 kDa | ||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3 | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||
References
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