Calnexin (ER Membrane Marker) Antibody [H16B4]

Catalog No.: F2880

    Home Primary Antibodies Calnexin (ER Membrane Marker) Antibody [H16B4]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000 - 1:5000
    1:4000
    1:360
    Application
    WB, IHC, FCM
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    68 kDa 90 kDa, 80 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    Calnexin (ER Membrane Marker) Antibody [H16B4] detects endogenous levels of total Calnexin protein.
    Clone
    H16B4
    Synonym(s)
    Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX
    Background
    Calnexin (ER Membrane Marker) is a type I integral membrane protein of the endoplasmic reticulum (ER) and a member of the lectin chaperone family that facilitates protein folding and quality control of nascent glycoproteins via its lectin-like luminal domain, which specifically binds to N-linked glycans on unfolded proteins. Calnexin has a transmembrane α-helix anchoring it to the ER membrane, a highly conserved ER luminal domain responsible for glycan recognition, and a cytosolic C-terminal domain subject to post-translational modifications such as phosphorylation and palmitoylation, which regulate its intracellular localization and interaction with cytosolic proteins. Calnexin forms a complex with ERp57, promoting proper disulfide bond formation in client proteins and preventing misfolding and aggregation, a key aspect of the calnexin/calreticulin cycle critical for maintaining ER proteostasis. Calnexin regulates ER-phagy by targeting misfolded glycoproteins to lysosomal degradation via interaction with the ER-phagy receptor FAM134B, thereby maintaining cellular homeostasis under ER stress conditions. Calnexin is also involved in the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways, preventing the accumulation of misfolded proteins linked to diseases like cystic fibrosis and neurodegeneration.
    References
    • https://pubmed.ncbi.nlm.nih.gov/39273068/
    • https://pubmed.ncbi.nlm.nih.gov/36766745/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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