Calnexin (ER Membrane Marker) Antibody [H16B4]

Catalog No.: F2880

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Biological Description

Specificity Calnexin (ER Membrane Marker) Antibody [H16B4] detects endogenous levels of total Calnexin protein.
Background Calnexin (ER Membrane Marker) is a type I integral membrane protein of the endoplasmic reticulum (ER) and a member of the lectin chaperone family that facilitates protein folding and quality control of nascent glycoproteins via its lectin-like luminal domain, which specifically binds to N-linked glycans on unfolded proteins. Calnexin has a transmembrane α-helix anchoring it to the ER membrane, a highly conserved ER luminal domain responsible for glycan recognition, and a cytosolic C-terminal domain subject to post-translational modifications such as phosphorylation and palmitoylation, which regulate its intracellular localization and interaction with cytosolic proteins. Calnexin forms a complex with ERp57, promoting proper disulfide bond formation in client proteins and preventing misfolding and aggregation, a key aspect of the calnexin/calreticulin cycle critical for maintaining ER proteostasis. Calnexin regulates ER-phagy by targeting misfolded glycoproteins to lysosomal degradation via interaction with the ER-phagy receptor FAM134B, thereby maintaining cellular homeostasis under ER stress conditions. Calnexin is also involved in the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways, preventing the accumulation of misfolded proteins linked to diseases like cystic fibrosis and neurodegeneration.

Usage Information

Application WB, IHC, FCM Dilution
WB IHC CHIP
1:1000 - 1:5000 1:4000 1:360
Reactivity Human
Source Rabbit Monoclonal Antibody MW 68 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/39273068/
  • https://pubmed.ncbi.nlm.nih.gov/36766745/

Application Data