ATP1A3 Antibody [M10F4]

Catalog No.: F2692

    Home Primary Antibodies ATP1A3 Antibody [M10F4]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:2000
    1:50-1:200
    1:400
    1 µg
    Application
    WB, IHC, IF, FCM
    Reactivity
    Bovine, Dog, Guinea pig, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Observed MW
    ~110 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    ATP1A3 Antibody [M10F4] detects endogenous levels of total ATP1A3 protein.
    Clone
    M10F4
    Synonym(s)
    ATPase, Na+/K+ transporting, alpha 3 polypeptide; ATPase, Na+K+ transporting, alpha 3 subunit; EC 3.6.3.9; Na(+)/K(+) ATPase alpha(III) subunit; Na(+)/K(+) ATPase alpha-3 subunit; AHC2; ATP1A1; ATP1A3; Atpa-2; ATPa1a3; CAPOS; DYT12; RDP
    Background
    ATP1A3, also known as the alpha-3 subunit of the Na⁺/K⁺-ATPase, is a critical ion pump protein predominantly expressed in neurons of the central nervous system. It maintains electrochemical gradients by actively transporting three sodium ions out of the cell and two potassium ions in, a process essential for sustaining neuronal excitability, signal transmission, and resting membrane potential. ATP1A3 is a transmembrane protein composed of multiple domains, including ion binding sites, ATP hydrolysis regions, and essential motifs for ion transport and pump activity, and is encoded by a gene with 23 exons. As the catalytic subunit of the larger Na⁺/K⁺-ATPase complex, ATP1A3 interacts with beta subunits such as ATP1B1, which contribute to pump stability and activity modulation. In addition to regulating synaptic vesicle cycling and cell volume, ATP1A3 influences various cellular processes. Mutations in ATP1A3 are associated with several neurological disorders, including Alternating Hemiplegia of Childhood (AHC), Rapid-Onset Dystonia-Parkinsonism (RDP), and other conditions characterized by disrupted ion gradients and neuronal dysfunction. Furthermore, ATP1A3 interacts with proteins involved in RNA translation and mitochondrial stability, suggesting broader roles in cellular metabolism and stress responses.
    References
    • https://pubmed.ncbi.nlm.nih.gov/33868146/
    • https://pubmed.ncbi.nlm.nih.gov/22842232/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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