Datasheet

Biological Description

Specificity	ATP1A3 Antibody [M10F4] detects endogenous levels of total ATP1A3 protein.
Background	ATP1A3, also known as the alpha-3 subunit of the Na⁺/K⁺-ATPase, is a critical ion pump protein predominantly expressed in neurons of the central nervous system. It maintains electrochemical gradients by actively transporting three sodium ions out of the cell and two potassium ions in, a process essential for sustaining neuronal excitability, signal transmission, and resting membrane potential. ATP1A3 is a transmembrane protein composed of multiple domains, including ion binding sites, ATP hydrolysis regions, and essential motifs for ion transport and pump activity, and is encoded by a gene with 23 exons. As the catalytic subunit of the larger Na⁺/K⁺-ATPase complex, ATP1A3 interacts with beta subunits such as ATP1B1, which contribute to pump stability and activity modulation. In addition to regulating synaptic vesicle cycling and cell volume, ATP1A3 influences various cellular processes. Mutations in ATP1A3 are associated with several neurological disorders, including Alternating Hemiplegia of Childhood (AHC), Rapid-Onset Dystonia-Parkinsonism (RDP), and other conditions characterized by disrupted ion gradients and neuronal dysfunction. Furthermore, ATP1A3 interacts with proteins involved in RNA translation and mitochondrial stability, suggesting broader roles in cellular metabolism and stress responses.

Specificity

ATP1A3 Antibody [M10F4] detects endogenous levels of total ATP1A3 protein.

Background

ATP1A3, also known as the alpha-3 subunit of the Na⁺/K⁺-ATPase, is a critical ion pump protein predominantly expressed in neurons of the central nervous system. It maintains electrochemical gradients by actively transporting three sodium ions out of the cell and two potassium ions in, a process essential for sustaining neuronal excitability, signal transmission, and resting membrane potential. ATP1A3 is a transmembrane protein composed of multiple domains, including ion binding sites, ATP hydrolysis regions, and essential motifs for ion transport and pump activity, and is encoded by a gene with 23 exons. As the catalytic subunit of the larger Na⁺/K⁺-ATPase complex, ATP1A3 interacts with beta subunits such as ATP1B1, which contribute to pump stability and activity modulation. In addition to regulating synaptic vesicle cycling and cell volume, ATP1A3 influences various cellular processes. Mutations in ATP1A3 are associated with several neurological disorders, including Alternating Hemiplegia of Childhood (AHC), Rapid-Onset Dystonia-Parkinsonism (RDP), and other conditions characterized by disrupted ion gradients and neuronal dysfunction. Furthermore, ATP1A3 interacts with proteins involved in RNA translation and mitochondrial stability, suggesting broader roles in cellular metabolism and stress responses.

Usage Information

Application

WB, IHC, IF, FCM

Dilution

WB	IHC	IF	FCM
1:2000	1:50-1:200	1:400	1 µg

Reactivity

Bovine, Dog, Guinea pig, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat

Source

Mouse Monoclonal Antibody

MW

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/33868146/
https://pubmed.ncbi.nlm.nih.gov/22842232/

ATP1A3 Antibody [M10F4]

Biological Description

Usage Information

References

Application Data