CAS No. 212135-62-1
Batch:
Purity:96.01
Chemical Structure
Check the
ZT-1a
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 5-chloro-N-[5-chloro-4-[(4-chlorophenyl)-cyanomethyl]-2-methylphenyl]-2-hydroxybenzamide | |
|---|---|---|
| CAS Number | 212135-62-1 | |
| Molecular Formula |
C22H15Cl3N2O2 |
|
| Molecular Weight (MW) | 445.73 | |
| InChIKey | RPTKRVXJNWPJLU-UHFFFAOYSA-N |
ZT-1a is a non-ATP-competitive kinase inhibitor of STE/SPS1-related proline-alanine-rich kinase (SPAK) with an ICof. By inhibiting SPAK, it reduces the phosphorylation of downstream cation-chloride cotransporters, including NKCC1 and KCCs, thereby regulating ion transport and cellular volume. Consequently, ZT-1a suppresses neuroinflammation, attenuates cerebral edema, and protects against neuronal tissue damage in models of ischemic brain injury. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
89 mg/mL
(199.67 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 4 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.