CAS No. 210344-95-9

Batch: Purity:98.44
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Z-DEVD-FMK Chemical Structure
Chemical Structure
Check the Z-DEVD-FMK product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-4-[[(2S)-4-methoxy-4-oxo-2-(phenylmethoxycarbonylamino)butanoyl]amino]-5-oxopentanoate
CAS Number 210344-95-9
Molecular Formula

C30H41FN4O12
Molecular Weight (MW) 668.66
SMILES CC(C)C(C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CCC(=O)OC)NC(=O)C(CC(=O)OC)NC(=O)OCC1=CC=CC=C1
InChIKey GBJVAVGBSGRRKN-JYEBCORGSA-N

Ordering Information

Biological Activity

Z-DEVD-FMK is a potent, cell-permeable, and irreversible caspase-3 inhibitor that also exhibits inhibitory activity against caspase-6, caspase-7, caspase-8, and caspase-10. By suppressing caspase-3 activation and catalytic activity, Z-DEVD-FMK blocks the execution phase of apoptosis, inhibiting downstream events such as poly(ADP-ribose) polymerase (PARP) cleavage and genomic DNA fragmentation across various cell types. Consequently, this compound serves as a valuable tool to elucidate caspase-dependent apoptotic signaling pathways. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (149.55 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.