CAS No. 429653-73-6
Batch:
Purity:99.24
Chemical Structure
Check the
Y16
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | (4E)-4-[[3-[(3-methylphenyl)methoxy]phenyl]methylidene]-1-phenylpyrazolidine-3,5-dione | |
|---|---|---|
| CAS Number | 429653-73-6 | |
| Molecular Formula |
C24H20N2O3 |
|
| Molecular Weight (MW) | 384.43 | |
| SMILES | CC1=CC(=CC=C1)COC2=CC=CC(=C2)C=C3C(=O)NN(C3=O)C4=CC=CC=C4 | |
| InChIKey | ITMLWGWTDWJSRZ-PXLXIMEGSA-N |
Y16 is an inhibitor of G-protein-coupled Rho guanine nucleotide exchange factors (GEFs) that blocks the interaction between leukemia-associated RhoGEF (LARG) and Rho (Kd = 80 nM). By preventing LARG-mediated Rho activation, Y16 lowers intracellular RhoA-GTP levels and blocks downstream Rho signaling pathways. Consequently, this disruption leads to suppressed cancer cell proliferation, migration, and invasive capacity in preclinical breast cancer models. [1]
|
Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
39 mg/mL
(101.44 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.