CAS No. 146986-50-7

Batch: Purity:99.92
Y-27632 Chemical Structure
Chemical Structure
Check the Y-27632 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide
CAS Number 146986-50-7
Molecular Formula

C14H21N3O

Molecular Weight (MW) 247.34
SMILES CC(N)C1CCC(CC1)C(=O)NC2=CC=NC=C2
InChIKey IYOZTVGMEWJPKR-VOMCLLRMSA-N

Ordering Information

Biological Activity

Y-27632 is a selective inhibitor of ROCK1 and ROCK2 with >200-fold selectivity over PKC, cAMP-dependent protein kinase, MLCK, and PAK. By inhibiting the ROCK signaling pathway, Y-27632 suppresses actomyosin contractility, alters cytoskeletal dynamics, and prevents smooth muscle contraction while promoting cell survival and neurite outgrowth in various cellular models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 49 mg/mL (198.1 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 49 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.