CAS No. 5119-48-2

Batch: Purity:99.92
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Withaferin A (WFA) Chemical Structure
Chemical Structure
Check the Withaferin A (WFA) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (1S,2R,6S,7R,9R,11S,12S,15R,16S)-6-hydroxy-15-[(1S)-1-[(2R)-5-(hydroxymethyl)-4-methyl-6-oxo-2,3-dihydropyran-2-yl]ethyl]-2,16-dimethyl-8-oxapentacyclo[9.7.0.02,7.07,9.012,16]octadec-4-en-3-one
CAS Number 5119-48-2
Molecular Formula

C28H38O6
Molecular Weight (MW) 470.60
InChIKey DBRXOUCRJQVYJQ-CKNDUULBSA-N

Ordering Information

Biological Activity

Withaferin A is a natural steroidal lactone that acts as an inhibitor of NF-κB activation and directly targets the intermediate filament protein vimentin. It prevents tumor necrosis factor-induced activation of IκB kinase β (IKKβ) via a thioalkylation-sensitive redox mechanism. Disruption of NF-κB pathway signaling suppresses pro-inflammatory responses and impairs endothelial cell proliferation, resulting in significant antitumor and antiangiogenic activities. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 94 mg/mL (199.74 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 4 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.