CAS No. 1977495-97-8

Batch: Purity:99.04
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Trilaciclib dihydrochloride Chemical Structure
Chemical Structure
Check the Trilaciclib dihydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-[[5-(4-methylpiperazin-1-yl)-2-pyridinyl]amino]spiro[1,3,5,11-tetrazatricyclo[7.4.0.02,7]trideca-2,4,6,8-tetraene-13,1'-cyclohexane]-10-one;dihydrochloride
CAS Number 1977495-97-8
Molecular Formula

C24H30N8O.2ClH
Molecular Weight (MW) 519.47
InChIKey BRCYOXKEDFAUSA-UHFFFAOYSA-N

Ordering Information

Biological Activity

Trilaciclib dihydrochloride is a potent inhibitor of CDK4/cyclin D1 (IC50 1 nM) and CDK6/cyclin D3 (IC50 4 nM). By selectively inhibiting CDK4/6 activity, it blocks retinoblastoma protein phosphorylation and transiently arrests hematopoietic stem and progenitor cells in the G1 phase of the cell cycle. This mechanism regulates cell proliferation and effectively protects bone marrow cells from chemotherapy-induced cytotoxic damage and myelosuppression. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 25 mg/mL
DMSO 1.3 mg/mL (2.5 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.