CAS No. 535-83-1

Batch: Purity:99.98
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Trigonelline Chemical Structure
Chemical Structure
Check the Trigonelline product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-methylpyridin-1-ium-3-carboxylate
CAS Number 535-83-1
Molecular Formula
C7H7NO2
Molecular Weight (MW) 137.14
SMILES C[N+]1=CC(=CC=C1)C([O-])=O
InChIKey WWNNZCOKKKDOPX-UHFFFAOYSA-N

Biological Activity

Trigonelline is a natural plant alkaloid and major bioactive constituent of coffee and fenugreek that acts as an inhibitor of FcεRI-mediated intracellular signaling pathways. It suppresses the phosphorylation of key signaling proteins including PLCγ1, PI3K, and Akt, while also inhibiting microtubule formation in RBL-2H3 cells. Through these molecular actions, trigonelline demonstrates potent anti-degranulation, anti-inflammatory, antioxidant, anti-diabetic, and neuroprotective activities. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 27 mg/mL
Ethanol 27 mg/mL
DMSO 10 mg/mL (72.91 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Shin SY, et al. J Agric Food Chem. 2013;61(51):12588-97.

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Note: Technical data last updated: Sep 1, 2026.