CAS No. 16858-02-9
Batch:
Purity:99.89
Chemical Structure
Check the
TPEN
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | N,N,N',N'-tetrakis(pyridin-2-ylmethyl)ethane-1,2-diamine | |
|---|---|---|
| CAS Number | 16858-02-9 | |
| Molecular Formula |
C26H28N6 |
|
| Molecular Weight (MW) | 424.54 | |
| SMILES | C(CN(CC1=NC=CC=C1)CC2=NC=CC=C2)N(CC3=NC=CC=C3)CC4=NC=CC=C4 | |
| InChIKey | CVRXLMUYFMERMJ-UHFFFAOYSA-N |
TPEN is a cell-permeable heavy metal chelator that selectively depletes intracellular zinc. Depletion of intracellular zinc by TPEN modulates zinc and nitric oxide signaling pathways, leading to increased intracellular reactive oxygen species production and DNA damage. These downstream molecular events trigger apoptosis and inhibit cell proliferation in leukemic and other cell models.
|
Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| Ethanol | 85 mg/mL |
| DMSO |
43 mg/mL
(101.28 mM)
; Warmed with 60°C water bath
; Ultrasonicated
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | 85 mg/mL |
| DMSO |
43 mg/mL
(101.28 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.