CAS No. 1971920-73-6

Batch: Purity:99.33
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Tolebrutinib Chemical Structure
Chemical Structure
Check the Tolebrutinib product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-amino-3-(4-phenoxyphenyl)-1-[(3R)-1-prop-2-enoylpiperidin-3-yl]imidazo[4,5-c]pyridin-2-one
CAS Number 1971920-73-6
Molecular Formula

C₂₆H₂₅N₅O₃
Molecular Weight (MW) 455.51
SMILES NC1=NC=CC2=C1N(C(=O)N2C3CCCN(C3)C(=O)C=C)C4=CC=C(OC5=CC=CC=C5)C=C4
InChIKey KOEUOFPEZFUWRF-LJQANCHMSA-N

Biological Activity

Tolebrutinib is an oral, CNS-penetrant, irreversible inhibitor of Bruton's tyrosine kinase (BTK) that potently targets BTK in Ramos B cells and microglial cells (IC. BTK signaling operates downstream of B-cell receptors and Fc receptors to regulate immune activation. Covalent inhibition of BTK blocks B-cell receptor signaling pathways, leading to suppressed B-cell activation and attenuated microglial inflammatory phenotypes. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 91 mg/mL (199.77 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 91 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Owens TD, et al. Clin Transl Sci. 2022;15(2):442-450.

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Note: Technical data last updated: Sep 1, 2026.