CAS No. 115532-52-0
Batch:
Purity:99.27
Chemical Structure
Check the
TMRE
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | [6-(dimethylamino)-9-(2-ethoxycarbonylphenyl)xanthen-3-ylidene]-dimethylazanium perchlorate | |
|---|---|---|
| CAS Number | 115532-52-0 | |
| Molecular Formula |
C26H27N2O3.ClO4 |
|
| Molecular Weight (MW) | 514.96 | |
| InChIKey | NBAOBNBFGNQAEJ-UHFFFAOYSA-M |
TMRE (tetramethylrhodamine ethyl ester) is a cell-permeant, lipophilic cationic fluorogenic dye used to evaluate mitochondrial membrane potential (ΔΨm). Driven by the negative electrical potential generated across the inner mitochondrial membrane during oxidative phosphorylation, TMRE selectively accumulates within active mitochondria. Loss or dissipation of ΔΨm during mitochondrial dysfunction or early apoptosis reduces intracellular TMRE fluorescence, providing a readout for cellular viability and cytochrome c release.[1][2]
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Concentration
Volume
Mass
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Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(194.18 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 100 mg/mL |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.