CAS No. 65-71-4

Batch: Purity:99.97
Thymine Chemical Structure
Chemical Structure
Check the Thymine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 65-71-4
Molecular Formula

C5H6N2O2

Molecular Weight (MW) 126.11
SMILES CC1=CNC(=O)NC1=O

Ordering Information

Biological Activity

Thymine is a naturally occurring pyrimidine nucleobase that serves as a fundamental building block of deoxyribonucleic acid (DNA). In double-stranded DNA, thymine pairs specifically with adenine via hydrogen bonds to maintain genomic integrity and double-helical structure. Thymine is involved in nucleic acid biosynthesis and cellular salvage pathways, playing an essential role in DNA replication, repair, and overall cellular proliferation.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 25 mg/mL (198.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.