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Thiazole Orange

Cat.No.E7454

Thiazole Orange is an asymmetric anthocyanin dye that can be coupled with oligonucleotides (ONs) to prepare fluorescent hybridization probes. This compound has been widely used in biomolecular detection and staining of DNA/ RNA in gels and can be used for reticulocyte analysis. It generates a significant fluorescence enhancement and high quantum yield when it binds with nucleic acids, especially RNA. This dye can permeate living cell membranes. It can use UV light for detection, but can also be detected with blue light. The excitation and emission of this chemical are λex = 510 nm (488 nm and 470 nm also show strong excitation) and λem = 527 nm, respectively.
Thiazole Orange Chemical Structure

Chemical Structure

Molecular Weight: 476.61

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Quality Control

Chemical Information, Storage & Stability

Molecular Weight 476.61 Formula Storage (From the date of receipt)
CAS No. 107091-89-4 -- Storage of Stock Solutions Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Mechanism of Action

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