CAS No. 30462-35-2

Batch: Purity:99.96
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Theaflavin 3,3'-digallate Chemical Structure
Chemical Structure
Check the Theaflavin 3,3'-digallate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name [2-[1-[5,7-dihydroxy-3-(3,4,5-trihydroxybenzoyl)oxy-3,4-dihydro-2H-chromen-2-yl]-3,4,6-trihydroxy-5-oxobenzo[7]annulen-8-yl]-5,7-dihydroxy-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate
CAS Number 30462-35-2
Molecular Formula

C43H32O20
Molecular Weight (MW) 868.7
InChIKey ZEASWHWETFMWCV-UHFFFAOYSA-N

Biological Activity

Theaflavin 3,3'-digallate is a black tea polyphenol that acts as a potent inhibitor of the Zika virus (ZIKV) NS2B-NS3 protease with an IC50 of 2.3 μM. By directly binding to the proteolytic cavity of ZIKV protease, it blocks the cleavage processing of viral polyprotein precursors. Consequently, this inhibition disrupts essential viral maturation processes, resulting in dose-dependent suppression of ZIKV replication and overall viral growth. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (115.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 100 mg/mL
Water 25 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Cui X, et al. Front Pharmacol. 2020;11:514313.

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Note: Technical data last updated: Sep 1, 2026.