CAS No. 51805-45-9

Batch: Purity:99.56
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TCEP Hydrochloride Chemical Structure
Chemical Structure
Check the TCEP Hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 51805-45-9
Molecular Formula

C9H16ClO6P
Molecular Weight (MW) 286.65
SMILES Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O

Biological Activity

TCEP Hydrochloride is a water-soluble, non-thiol reducing agent that selectively cleaves disulfide bonds in proteins and peptides. By maintaining sulfhydryl groups in a reduced state, TCEP modulates redox-sensitive signaling pathways and promotes NF-κB-DNA binding in a dose-dependent manner. This reduction of critical cysteine residues preserves protein stability and activity, supporting downstream cellular functions and transcriptional regulation in biochemical assays. [1]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 57 mg/mL (198.84 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 57 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.

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Reference

[1] Getz EB, et al. Anal Biochem. 1999;273(1):73-80.

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Note: Technical data last updated: Sep 1, 2026.